The innate immune response is a crucial component of early resistance to infection, and it is now revealing increasing levels of complexity. The ability to modify the genome in vivo, has facilitated understanding of complex interactions between leucocytes and other components of the immune system, and phenotype-driven strategies using chemical mutagenesis have placed another powerful weapon in the armamentarium. In, Leucocytes: Methods and?Protocols?provides detailed protocols and practical advice on a variety of modern approaches to the study of leucocytes and their products.? Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Leucocytes: Methods and Protocols seeks to aid scientists in further study into the immune system and leucocytes.
The 3rd edition of this Methods in Molecular Biology book offers detailed protocols and practical advice on modern approaches to the study of leucocytes and their products. Includes materials lists, step-by-step lab protocols, and key tips on troubleshooting.
1. ENU Based Phenotype Driven Screening
Vera? M. Ripoll, Philip L. Kong, and Paul K. Potter
2. Detection and Quantification of Cytokines and Other Biomarkers
Evan L. Chiswick, Elizabeth Duffy, Brian Japp and Daniel Remick
3. Flow Cystometry Analysis of Cell Cycling and Proliferation in Mouse Hematopoetic Stem and Progenitor Cells
Val?rie Barbier, Bianca Nowlan, Jean-Pierre L?vesque and Ingrid G. Winkler
4. Flow Cytometry Measurement of Bone Marrow Perfusion in the Mouse and SlĂ
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