Lipases and Phospholipases Methods and Protocols [Paperback]

Lipases are the most applied enzymes in organic synthesis due to their broad substrate acceptance and because of the availability of the molecular, biochemical, themodynamical and solvent engineering tools, which allows the optimization of lipases and lipase-catalyzed reactions. On the other hand, phospholipases are emerging as useful enzymes in food and pharmaceutical industries.?In Lipases and Phospholipases: Methods and Protocols, expert researchers in the field provide key techniques to investigate these essential enzymes. Focusing on fundamental issues, current and new applications as well as practical step-by-step protocols, and the extensive applications of lipases and the potential application of phospholipases and its inhibitors. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

?Thorough and intuitive, Lipases and Phospholipases: Methods and Protocols aids scientists in continuing to study lipases, phopholipases and related enzymes.

This book details fundamental issues and new applications of lipases and phospholipases. It? provides step-by-step detail essential for reproducible results and contains key notes and implementation advice from the experts.

Part I: Overview of Lipases, Phospholipases and Related Enzymes

1. Lipases: An Overview????????????????????????????????????????????????????????????????? Leticia Casas Godoy, Sophie Duquesne, Florence Bordes, Georgina Sandoval and Alain Marty

2. Lipases or Esterases: Does it Really Matter? Toward a New Bio-Physico-Chemical Classification?????????????????????????????????????????????? Yassine Ben Ali, Robert Verger, and Abdelkarim Aboul“+