Lipidomics [Hardcover]

This volume details methods for the analyses of specific lipid classes and lipidomics analyses of cells such as lymphocytes and oocytes.  Lipidomics guides readers through chapters on direct-flow and chromatographic methods (SFC, UHPLC, HPTLC, ion-mobility);  derivatization methods for lipids (amines, fatty aldehydes and ketones); TOF-SIMS imaging of lipids; and characterization of lipid transfer proteins. Additional chapters also provide an authoritative overview of lipidomics strategies and a detailed review of high-resolution mass spectrometric methods are included in this volume. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.

Concise and easy-to-use, Lipidomics aims to ensure successful results in the further study of this vital field.

1. Introduction and Overview of Lipidomic Strategies

William J. Griffiths and Yuqin Wang

 

2. Non-targeted Lipidomics Utilizing Constant Infusion High Resolution ESI Mass Spectrometry

Paul L. Wood

 

3. Ultrahigh-Resolution Lipid Analysis with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Mark R. Emmett and Cheryl F. Lichti

 

4. MALDI-Imaging Mass Spectrometry of Brain Lipids

Norelle C. Wildburger

 

5. Ion-Mobility Mass Spectrometry for Lipidomics Applications

 

6. In vitro Assay to Extract Specific Lipid Types from Phospholipid Membranes using Lipid-Transfer Proteinl#S