Listeria monocytogenes Methods and Protocols [Hardcover]

Listeria monocytogenes is still a major threat to public health. A new book in the Methods in Molecular Biology series, Listeria monocytogenes: Methods and Protocols addresses its titular pathogen with protocols and methodologies used in research to gain a better understanding of Listeria at a molecular level. The topics covered include sampling in order to isolate Listeria, methods for their identification and characterization, methods for gene manipulation and finally, methods for control of the organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Vital and authoritative, Listeria monocytogenes: Methods and Protocols aims to contribute to the harmonization of the methods used in the field and will therefore benefit all those interested in Listeria research.

Part I: Detection, Quantification, and Confirmation

1. Sampling the Production/Processing Environment for Listeria

??????????? Anca Ioana Nicolau and Andrei Sorin Bolocan

2. Traditional Methods for Isolation of Listeria monocytogenes

??????????? Rui Magalh?es, Cristina Mena, V?nia Ferreira, Gon?alo Almeida, Joana Silva, and Paula Teixeira

3. Confirmation of Isolates of Listeria by Conventional and Real-Time PCR

??????????? David Rodr?guez-L?zaro and Marta Hern?ndez

Part II: Characterization and Typing

4. Serotype Assignment by Sero-Agglutination, ELISA, and PCR

??????????? Lisa Gorski