Liver Proteomics Methods and Protocols [Hardcover]

The liver is responsible for a wide range of critical functions essential to life, and is composed of several different cell types. In? Liver Proteomics: Methods and Protocols, expert researchers in the field detail many of the methods that are used to study the live. These methods include the ?most up-to-date strategies being used to characterize the liver proteome at the global, cellular, subcellular, post translational and functional level.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

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Authoritative and practical, Liver Proteomics: Methods and Protocols seeks to aid scientists in the further study of this crucially important organ.

The liver governs many critical life functions and has several distinct cell types. This guide to characterizing liver proteome at every level, from global to subcellular and post-transitional, features laboratory-ready protocols and invaluable expert advice.

1. Cell Surface Capturing (CSC) Technologies for the Surfaceome Discovery of Hepatocytes

Damaris Bausch-Fluck, Hofmann Andreas, and Bernd Wollscheid

2. An On-Target Desalting and Concentration Sample Preparation Protocol for MALDI-MS and MS/MS Analysus

Xumin Zhang, Quanhui Wang, Xiaomin Lou, Haidan Sun, Peter Roepstorff, and Siqi Liu

3. Plasma Membrane Isolation using Immobilized Concanavalin A Magnetic Beads

Yu-Chen Lee, Martina Srajer Gajdoski, Djuro Josic, and Sue-Hwa Lin

4.? Analysis of the Cattle Liver Proteome by High-sensitive Liquid Chromatography Coupled with Mass Spectl£&