Long Non-Coding RNAs Methods and Protocols [Paperback]

This volume assembles a broad spectrum of methods used in long non-coding RNAs (lncRNA) research, ranging from computational annotation of lncRNA genes to molecular and cellular analyses of the function of individual lncRNA. Long Non-Coding RNAs: Methods and Protocols also discusses methods used to study circular RNAs and RNA splicing, as well as influential findings on lncRNA in human diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Long Non-Coding RNAs: Methods and Protocols is a must-have for molecular biologists, cell and developmental biologists, specialists who conduct disease-oriented research, and bioinformatics experts who seek a better understanding on lncRNA expression and function by computational analysis of the massive sequencing data that are rapidly accumulating in recent years.

1.?????? LncRNA Pulldown Combined with Mass Spectrometry to Identify the Novel LncRNA-Associated Proteins
Zhen Xing, Chunru Lin, and Liuqing Yang

2.?????? Cross-Linking Immunoprecipitation and qPCR (CLIP-qPCR) Analysis to Map Interactions between Long Non-Coding RNAs and RNA-Binding Proteins
Je-Hyun Yoon and Myriam Gorospe

3.?????? Characterization of Long Non-Coding RNA Associated Proteins by RNA-Immunoprecipitation

Youyou Zhang, Yi Feng, Zhongyi Hu, Xiaowen Hu, Chao-Xing Yuan, Yi Fan, Lin Zhang

4.?????? Isolation of Protein Complexes Associated with Long Non-Coding RNAs
Kiranmai Gumireddy, Jinchun Yan, and Qihong Huang