Membrane bioenergetics is one of the most rapidly growing areas within physico-chemical biology. Main aspects treated in this book include energy conservation and utilization by membrane-linked molecular mechanisms such as intracellular respiration, photosynthesis, transport phenomena, rotation of bacterial flagella, and the regulation of heat production.Membrane bioenergetics is one of the most rapidly growing areas within physico-chemical biology. Main aspects treated in this book include energy conservation and utilization by membrane-linked molecular mechanisms such as intracellular respiration, photosynthesis, transport phenomena, rotation of bacterial flagella, and the regulation of heat production.1 Introduction.- 1.1 A Biology Building and the Place of Bioenergetics.- 1.2 Essential Definitions.- 1.2.1 Energy-Transducing Membranes.- 1.2.2 Coupling Ions.- 1.2.3 Convertible Energy Currencies of the Living Cell.- 1.3 ???H, ?p, ???Na and ?s.- 1.4 Adenosine Triphosphate.- 1.5 Membrane Lipids.- 1.6 Lipid Bilayer.- 1.7 Membrane Proteins.- 2 Specific Methods of Membrane Bioenergetics.- 2.1 Membrane Potential Measurement.- 2.1.1 Proteoliposomes.- 2.1.2 Direct ?? Measurement in the Proteoliposome-Collodion Film System.- 2.1.3 ?? Measurement in Intact Cells and Organelles.- 2.1.3.1 Microelectrode Techniques.- 2.1.3.2 Natural Penetrating Ions and Ionophores.- 2.1.3.3 Synthetic Penetrating Ions.- 2.1.3.4 Fluorescing Penetrating Ions: A ?? Monitoring in a Single Cell or Organelle.- 2.1.3.5 The Carotenoid Shift.- 2.2 ?pH Measurement.- 2.3 Measurement of Fast H+ Dissociation-Association.- 3 Primary ???H Generators.- 3.1 The Cyclic Photoredox Chain of Purple Bacteria.- 3.1.1 The Main Components and the Principle of Their Function.- 3.1.2 The Reaction Centre Complex.- 3.1.2.1 The Protein Composition.- 3.1.2.2 The Arrangement of Redox Groups.- 3.1.2.3 The Sequence of Electron Transfer Events.- 3.1.2.4 The Mechanism of ???H Generation.- 3.1.3 The CoQH2-Cytochrorne c Reductase.l