Membrane Proteins Production for Structural Analysis [Hardcover]

This book updates the latest development in production, stabilization and structural analysis techniques of membrane proteins. This field has made significant advances since the elucidation of the first 3-D structure of a recombinant G Protein Coupled Receptor (GPCR), rhodopsin, with the structure of several more GPCRs having been solved in the past five years. In fact, the 2012 Nobel Prize in Chemistry was awarded for groundbreaking discoveries on the inner workings of GPCRs. This book is essential reading for all researchers, biochemists and crystallographers?working with membrane proteins, who are interested by the structural characterization of their favorite protein and who wish to follow the expression, migration, modifications and recycling of a membrane protein.

.- 1 Membrane Proteins Production for Structural Analysis

.- Isabelle Mus-Veteau, Pascal Demange, Francesca Zito

.- 2? Membrane Protein Quality Control in Cell-Free Expression Systems: Tools, Strategies and Case Studies?

.- Davide Proverbio, Erik Henrich, Erika Orb?n, Volker D?tsch and Frank Bernhard

.- 3? Bacterial Expression and Stabilization of GPCRs

.- Jean Louis Ban?res

.- 4? Membrane Protein Production in Escherichia Coli: Overview and Protocols

.- Georges Hattab, Annabelle Y. T. Suisse, Oana Ilioaia, Marina Casiraghi, Manuela Dezi, Xavier Warnet, Dror Warschawski, Karine Moncoq, Manuela Zoonens and Bruno Miroux

.- 5? Lactococcus Lactis, Recent Developments in Functional Expression of Membrane Proteins

.- Sana Bakari, Fran?ois Andr?, Daphn? Seigneurin-Berny, Marcel Delaforge, Norbert Rolland and Annie Frelet-ló+