Membrane Trafficking [Paperback]

As membrane trafficking research has expanded over the past thirty years, a remarkable convergence of information has been gained by using genetic approaches in yeast cells with biochemical approaches in mammalian cells. This book reflects these advances by devoting one section of the book to yeast cells and the other to mammalian cells, with each section providing both classic and cutting-edge techniques to study macromolecular transport across the membranes.

As membrane trafficking research has expanded over the past thirty years, a remarkable convergence of information has been gained by using genetic approaches in yeast cells with biochemical approaches in mammalian cells. Membrane Trafficking reflects these advances by devoting one section of the book to yeast cells and the other to mammalian cells, with each section providing both classic and cutting-edge techniques to study macromolecular transport across the membranes. These protocols, contributed by top experts in the field, have been tested in laboratories all around world. As a volume of the highly successful Methods in Molecular Biology series, the chapters include step-by-step laboratory protocols, a list of necessary materials and reagents, and the greatly beneficial Notes section, which aids in troubleshooting and avoiding known pitfalls.

Comprehensive and state-of-the-art, Membrane Trafficking is the ideal reference for both senior researchers experienced in the field and graduate students studying membrane trafficking for the first time.

Table of Contents Part I. Yeast Cells Ch. 1) Freight Management in the Cell: Current Aspects of Intracellular Membrane Trafficking Johannes M. Herrmann and Anne Spang Ch. 2) High-throughput Protein Extraction and Immunoblotting Analysis in Saccharomyces cerevisiae Todd C. Lorenz, Vikram C. Anand, and Gregory S. Payne Ch. 3) Genome-wide Analysis of Membrane Transport Using Yeast Knockout Arrays Helen E. Burstl3%