Mesenchymal Stem Cells Methods and Protocols [Paperback]

For over forty years, mesenchymal stem cells (MSCs) have been scrutinized and studied, garnering much attention due to their broad therapeutic efficacy. In this essential book, leaders in the field were assembled to contribute detailed methodologies for the isolation and characterization of human and rodent MSCs. Cutting edge and easy to use, this book is the perfect resource for scientists attempting to pursue this important and ever-developing field of research.

For over forty years, mesenchymal stem cells (MSCs) have been scrutinized and studied, garnering much attention due to their broad therapeutic efficacy. In Mesenchymal Stem Cells: Methods and Protocols, leaders in the field were assembled to contribute detailed methodologies for the isolation and characterization of human and rodent MSCs. Recently, these vital cells have shown therapeutic benefits in the treatment of myocardial infarction, stroke, lung diseases, spinal cord injury and other neurological disorders, thus promising a boundless future in their study.

Cutting edge and easy to use, Mesenchymal Stem Cells: Methods and Protocols is the perfect resource for scientists attempting to pursue this important and ever-developing field of research.

Table of Contents: Preface. Darwin J. Prockop, Donald G. Phinney, Bruce A. Bunnell Part I: Isolation of human multipotential stromal cells from bone marrow and adipose tissue. Chapter 1. Isolation and culture of bone marrow-derived human multipotent stromal cells (hMSCs). Margaret Wolfe, Radhika Pochampally, William Swaney and Roxanne Reger Chapter 2. Mesenchymal stem cells from adult bone marrow. Mark F. Pittenger Chapter 3. A method to isolate and purify human bone marrow stromal stem cells. Stan Gronthos and Andrew C. W. Zannettino Chapter 4. Adipose-derived stem cells. John K. Fraser, Min Zhu, Isabella Wulur and Zeni Alfonso Chapter 5. Isolation of human adipose-derived stem cells from biopsies anlÎ