Metabolic Profiling Methods and Protocols [Hardcover]

At the intersection of metabolite analysis, metabolic fingerprinting, and metabolomics, the study of metabolic profiling has evolved steadily over the course of time as have the methods and technologies involved in its study. In Metabolic Profiling: Methods and Protocols, expert researchers in the field present protocols that are illustrative of the evolution of metabolic profiling from single molecule analysis to global metabolome profiling. Comprised of the most essential techniques, this volume covers topics from inborn errors of metabolism and drug metabolite analysis to nuclear magnetic resonance metabolic profiles. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Metabolic Profiling: Methods and Protocols serves as a resource for both established and new investigators in this vital and ever-developing field.

This book presents protocols that are illustrative of the evolution of metabolic profiling from single molecule analysis to global metabolome profiling.? It features cutting-edge protocols, presented from the perspective of leading experts in the field.

1. Origins of Metabolic ProfilingArthur B. Robinson and Noah E. Robinson2. Amino Acid Profiling for the Diagnosis of Inborn Errors of MetabolismMonique Piraud, S?verine Ruet, Sylvie Boyer, C?cile Acquaviva, Pascale Clerc-Renaud, David Cheillan, and Christine Vianey-Saban3. Acylcarnitines: Analysis in Plasma and Whole Blood Using Tandem Mass SpectrometryDavid S. Millington and Robert D. Stevens4. Analysis of Organic Acids and Acylglycines for the Diagnosis of Related Inborn Errors of Metabolism by GC- and HPLC-MSGiancarlo la Marca and Cristiano Rizzo5. HPLC Analysis of Purines and Pyrimidines for the Clinical Bioclƒ<