Microbial Metabolic Engineering Methods and Protocols [Hardcover]

Metabolic engineering is the practice of genetically optimizing metabolic and regulatory networks within cells to increase production and/or recovery of certain substance from cells. In Microbial Metabolic Engineering: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study metabolic engineering. These include methods and techniques to engineer genes and pathways, use of modern biotechnology tools in microbial metabolic engineering, and examples of metabolic engineering for real world applications such as whole cell biosensors and acetate control in large scale fermentation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

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Authoritative and practical, Microbial Metabolic Engineering: Methods and Protocols seeks to provide researchers with an overview of key topics on microbial metabolic engineering.

Table of Contents

Preface

Preamble

1??????? Screening for Cellulases with Industrial Value and their use in Biomass Conversion

Julia J?rgensen, Nele Ilmberger and Wolfgang R. Streit

2??????? Reversal of NAD(P)H Cofactor Dependence by Protein Engineering

Sabine Bastian and Francis H. Arnold

3??????? Quantifying Plasmid Copy Numberlcl