Microbial Toxins Methods and Protocols [Paperback]

This second edition volume expands on the previous editions with detailed protocols on (cyano) bacterial and mold fungus toxins, and a specific focus on Gram-negative endotoxins (lipopolysaccharides). The chapters in Microbial Toxins: Methods and Protocols, Second Edition present techniques that cover topics such as detection of cholera toxin by an immunochromatographic test strip; label-free immune-sensors for the fast detection of Listeria in food; endotoxin removal from Escherichia coli bacterial lysate using biphasic liquid system; and micromethods for isolation and structural characterization of lipid A, and polysaccharide regions of bacterial lipopolysaccharides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Microbial Toxins: Methods and Protocols, Second Edition is a valuable and useful resource for scientists who are interested in this field, and is a great tool for researchers who are looking to learn about new, particular techniques to further enhance their work.

Preface...

Table of Contents...

Contributing Authors....

1. Detection of Cholera Toxin by an Immunochromatographic Test Strip
Eiki Yamasaki, Ryuta Sakamoto, Takashi Matsumoto, Biswajit Maiti, Kayo Okumura, Fumiki Morimatsu, G. Balakrish Nair, and Hisao Kurazono

 2. Electrochemical Aptamer Scaffold Biosensors for Detection of Botulism and Ricin Proteins
Jessica Daniel, Lisa Fetter, Susan Jett, Teisha J. Rowland, and Andrew J. Bonham