Microbial-Based Biopesticides Methods and Protocols [Paperback]

This volume focuses on the developmental areas of biopesticides: production, formulation, application and field efficacy. Chapters guide readers through methods and techniques on environmental, mammalian, safety, and registration.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Microbial-Based Biopesticides aims to ensure successful results in the further study of this vital field.

1 What are Microbial-based Biopesticides?

Mar?a E. Mor?n-Diez and Travis R. Glare

 

Part I: Screening, Isolation and Identification of Potential Biological Control Agents

2. Isolation and Mass Production of Trichoderma

Artemio Mendoza-Mendoza, Annabel Clouston, Jin-Hua Li, Maria Fernanda Nieto-Jacobo, Nicholas Cummings, Johanna Steyaert,  and Robert Hill

 

Part II: Evaluating Mode of Action of Microorganisms

3. Methods for the Evaluation of the Bioactivity and Biocontrol Potential of Species of Trichoderma

Johanna Steyaert, Emily Hicks, Janaki Kandula, Diwakar Kandula, Hossein Alizadeh, Mark Braithwaite, Jessica Yardley, and Artemio Mendoza-Mendoza

 

Part III:  Mass Production and Formulations: Bacteria

4. Purification of the Yersinia Entomophaga Yen-TC Toxin Complex using Size Exclusion Chromatography

Sandra A. Jones and Mark R. H. Hurst