Microfluidic and Compartmentalized Platforms for Neurobiological Research [Hardcover]

This volume presents cutting-edge techniques to design, fabricate and use compartmentalized microfluidic devices. Chapters encompass axon guidance and manipulation, compartmentalized devices for synapse manipulation, cell populations interaction either in physiological or pathological condition and compartmentalized devices for pharmacological research and drug discovery. Written in the popular?Neuromethods?series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.

Authoritative and practical,?Microfluidic and Compartmentalized Platforms for Neurobiological Research?provides practical skills needed to fabricate, use microfluidics and compartmentalized platforms with neuronal cell cultures as well as the strengths of these exciting devices and their precious contribution in the field of Neuroscience.

PART I: Designs and Methods

1.????? The Fabrication of Microfluidic Platforms with Pneumatically/Hydraulically Controlled PDMS Valves and their use in Neurobiological Research

?Bryson M. Brewer, Donna J. Webb and Deyu Li

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2.????? A Reliable Reversible Bonding Method for Perfused Microfluidic Devices

Paola Occhetta, Emilia Biffi, and Marco Rasponi

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3.????? Bridging Two Cultures: Minimalistic Networks Prepared by Microfluidic Arraying, and Open Access Compartments for Electrophysiology

Jonathan West, Ngoc-Duy Dinh, Heike Hardelauf, Ya-Yu Chiang, Tracey A. Newman, Mariana Vargas-Caballero, Ayodeji A. Asuni, Katrin Deinhardt, and Martin Arundell

PART II: Axonal Guidance and Manipulation

4.????? Asymmetric Genetic Manipulation and Patch Clamp Recording of Neurons in a Microfl#p