Microinjection Methods and Protocols [Hardcover]

This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impact our society in general. The opening half of the book focuses on methods for generating mouse models, as they are still the most popular in genome engineering research, while the second half examines gene-editing in a variety of other species, opened up by the developments in ZFN, TALEN, and CRISPR techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

1. Microinjection and Micromanipulation: A Historical Perspective

            Wenhao Xu

 

2. Production of Transgenic Mice by Pronuclear Microinjection

            Xin-an Pu, Anthony P. Young, and H. Michael Kubisch

 

3. Transgene Recombineering in Bacterial Artificial Chromosomes

            Michael G. Zeidler and Thomas L. SaundelÎ