MicroRNA and Cancer Methods and Protocols [Hardcover]

This volume details basic principles of experimental and computational methods for the study of microRNAs in cancer research and, therefore, provides a firm grounding for those who wish to develop further applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, MicroRNA and Cancer: Methods and Protocols, Second Edition?aims to ensure successful results in the further study of this vital field

1. Non- coding RNAs in DNA Damage Response: Opportunities for Cancer Therapeutics

Wani Arjumand, Asia Asiaf, and Shiekh Tanveer Ahmad

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2. MicroRNAs in Breast Cancer: Diagnostic and Therapeutic Potential

Asia Asiaf, Shiekh Tanveer Ahmad, Wani Arjumand, and Mohammad Afzal Zargar

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3. Involvement of miRNAs and Pseudogenes in Cancer

L?tfi TUTAR, Aykut ?ZG?R, and Yusuf TUTAR

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4. microRNAs Reprogram Tumor Immune Response

Wenfang Cheng and Wei Wu

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5. APOBEC Gene Expression Regulated by microRNAs

Wei Cao and Wei Wu

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6. MicroRNAs Changes the Landscape of Cancer Resistance

Jun Zhu and Wei Wu

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7. MicroRNA, Noise, and Gene Expression Regulation

Wei Wu

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PART II: Protocol Section

8. Deep Sequencing Reveals a microRNA Expression Sl“ž