MicroRNA Protocols [Paperback]

MicroRNA Protocols provides diverse, novel, and useful descriptions of miRNAs in several species, including plants, worms, flies, fish, chicks, mice, and humans. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the identification of miRNAs. This volume will stimulate the reader to explore diverse ways to understanding the mechanism in which miRNAs facilitate the molecular aspects of the biomedical research.MicroRNA Protocols provides diverse, novel, and useful descriptions of miRNAs in several species, including plants, worms, flies, fish, chicks, mice, and humans. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the identification of miRNAs. This volume will stimulate the reader to explore diverse ways to understanding the mechanism in which miRNAs facilitate the molecular aspects of the biomedical research.The MicroRNA: Overview of the RNA Gene That Modulates Gene FunctionsShao-Yao Ying, Donald C. Chang, Joseph D. Miller, and Shi-Lung LinStructure Analysis of MicroRNA PrecursorsJacek Krol and Wlodzimierz J. KrzyzosiakMicroRNA Biogenesis: Isolation and Characterization of the Microprocessor ComplexRichard I. Gregory, Thimmaiah P. Chendrimada, and Ramin ShiekhattarRecognition and Cleavage of Primary MicroRNA TranscriptsYan Zeng and Bryan R. CullenMouse Embryonic Stem Cells as a Model Genetic System to Dissect and Exploit the RNA Interference MachineryStefan A. Muljo and Chryssa KanellopoulouMicroRNAs and Messenger RNA TurnoverJulia A. Chekanova and Dmitry A. BelostotskyPrediction of MicroRNA TargetsMarc RehmsmeierPrediction of Human MicroRNA TargetsBino John, Chris Sander, and Debora S. MarksComplications in Mammalian MicroRNA Target PredictionNeil R. Smalheiser and Vetle I. TorvikmiRBase:The MicroRNA Sequence DatabaseSam Griffiths-JonesMethodologies for High-Throughput Expression ProfillS²