Microscopy Techniques [Hardcover]

This review series covers trends in modern biotechnology, including all aspects of this interdisciplinary technology, requiring knowledge, methods, and expertise from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science.

With contributions by numerous experts

1 Miyawaki, T. Nagai, H. Mizuno: Engineering Fluorescent Proteins.- 2 C.-K. Sun: Higher Harmonic Generation Microscopy.- 3 R. Gr?f, J. Rietdorf, T. Zimmermann: Live Cell Spinning Disk Microscopy.- 4 T. Wazawa, M. Ueda: Total Internal Reflection Fluorescence Microscopy in Single Molecule Nanobioscience.- 5 T. Kohl, P. Schwille: Fluorescence Correlation Spectroscopy with Autofluorescent Proteins.- 6 E.B. van Munster, T.W.J. Gadella: Fluorescence Lifetime Imaging Microscopy.- 7 A.B. Houtsmuller: Fluorescence Recovery After Photobleaching: Application to Nuclear Proteins.- 8 J.-B. Sibarita: Deconvolution Microscopy.- 9 T. Zimmermann: Spectral Imaging and Linear Unmixing in Light Microscopy.- K. Miura: Tracking Movement in Cell Biology.-

From the reviews:

Light Microscopy has returned to fashion through the synergy of green fluorescent protein and a clutch of techniques involving some permutation of lasers, mathematics and acronyms, which offer the prospect of watching biological molecules at work inside living cells. This volume provides an introduction to these techniques & . this volume will be a longer-term reference for anyone who wants to understand how these methods actually work. In this respect it is excellent. (John Armstrong, Microbiology Today, July, 2006)

Advances in microscopy techniques have recently had a tremendous impact on research in biochemistry and molecular biology. While the study of biochemical reactions was formerly confined to cuvette-based measurements of purified biomolecules, it is now possible for investigators to make such analyses inside the complex envirlS'