This detailed volume collects chapters that seek to expand our knowledge of molecular events and extended molecular networks in gene regulation. The contents explore numerous aspects of miRNA biogenesis, a sophisticated series of events that assure a finely tuned regulation of miRNA expression and activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, miRNA Biogenesis: Methods and Protocols aims to contribute to the further development of knowledge and application of miRNA biogenesis, both in the basic research lab and in the clinic.
Chapter 1 microRNA biogenesis Review
Chapter 2 Identifying microRNA transcription sites
Chapter 3 Annotating primary microRNAs
Chapter 4 Defining Microprocessor co-factors
Chapter 5 Endogenous assay for primary microRNA processing
Chapter 6 In vitro assay for primary microRNA processing
Chapter 7 miRtrons
Chapter 8 Inhibiting primary microRNA processing
Chapter 9 &lc<
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