Mitochondria Practical Protocols [Hardcover]

This volume compiles a broad range of step-by-step protocols, complementary to the ones published in the first edition of this book, to study various aspects of mitochondrial structure and function in different model organisms, both in vitro and in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Mitochondria: Practical Protocols, Second Edition aims to be useful for beginners as well as for experienced researchers in the field.

A Guide to Computational Methods for Predicting Mitochondrial Localization.- Isolation of Functional Mitochondria from Cultured Cells and Mouse Tissues.- Isolation of Mitochondria From Saccharomyces Cerevisiae.- Isolation of Contact Sites Between Inner and Outer Mitochondrial Membranes.- Isolation Of Mitochondria-Associated Membranes (MAM) from Mouse Brain Tissue.- Label-Free Quantitative Analysis of Mitochondrial Proteomes using the Multienzyme Digestion-Filter Aided Sample Preparation (MED-FASP) and Total Protein Approach.- Quantitative Analysis of Glycerophospholipids in Mitochondria by Mass Spectrometry.- Detection of Cysteine Redox States in Mitochondrial Proteins in Intact Mammalian Cells.- Chemical Crosslinking in Intact Mitochondria.- Reconstitution of Mitochondrial Membrane Proteins into Nanodiscs by Cell-Free Expression.- Detection of Dual Targeting and Dual Function of Mitochondrial Proteins in Yeast.- Localizing Mrnas Encoding Mitochondrial Proteins in Yeast by Fluorescence Microscopy and Subcellular Fractionation.- Assessing Mitochondrial Bioenergetics in Isolatlœ