This second edition volume expands on the previous edition with chapters discussing the latest developments and research initiatives in mitochondrial functions. The chapters in this book explore topics such as high-resolution fluorespirometry and OXPHOS protocols in human cells, analysis of mitochondrial oxygen consumption, mitochondrial bioenergetics, and mitochondrial dynamics in mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Mitochondrial Bioenergetics: Methods and Protocols, Second Edition is a valuable resource for students, and practitioners in the pharmaceutical sciences, environmental sciences, and mitochondrial genetics industries.
Preface&
Table of Contents&
Contributing Authors&
1.Overview of Mitochondrial Bioenergetics
Vitor M.C. Madeira
2.Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal Cells
Ana M. Silva and Paulo J. Oliveira
3.High-Resolution FluoRespirometry and OXPHOS Protocols for Human Cells, Permeabilized Fibres from Small Biopsies of Muscle and Isolated Mitochondria
Carolina Doerrier, Luiz F. Garcia-Souza, Gerhard Krumschnabel, Yvonne Wohlfarter, Andr?s T. M?sz?ros, and Erich Gnaiger
4.High-Throughput Analysis of Mitochondrial Oxygen Consumption