Mitochondrial Regulation Methods and Protocols [Paperback]

In this volume expert researchers in the field detail the many methods for further research into regulation of mitochondrial function.?Chapters focus on mitochondria with other cellular components, discussing how these interactions influence the dynamics of mitochondrial structure and biogenesis. Written in the highly successful Methods in Molecular Biology?series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Mitochondrial Regulation: Methods and Protocols seeks to aid advanced undergraduates, graduates, postgraduates, and beginning researchers in the areas of molecular and cellular biology, biochemistry and bioenergetics.

1. Isolation of Crud Mitochondrial Fraction from Cells

Mariusz R. Wieckowski and Lech Wojtczak

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2. Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein  Analyses of MicroRNA and Protein Expressions

Pedro M. Borralho, Clifford J. Steer, and Cec?lia M. P. Rodrigues

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3. PCR Based Determination of Mitochondrial DNA Copy Number in Multiple Species

Rooney JP, Ryde IT, Sanders LH, Howlett EH, Colton MD, Germ KE, Mayer GD, Greenamyre JT, and Meyer JN

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4. NAD⁺ Content and its Role in Mitochondria

Wei Li and Anthony A. Sauve

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5. Measuring PGC-1? and its Acetylation Status in Mouse Primary Myotubes

Ana P. Gomes and David A. Sinclair

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6. Measurement of Mitochondrial Oxygen Consumption Rls’