The Molecular and Cellular Biology of Fertilization [Paperback]

Prologue.- 1 Fertilization and Immunity-Revisited.- Summary.- Univalent Antibodies.- Paramecium: A Model System.- References.- 2 Sperm-Egg Interactions Preparatory to Fertilization.- Summary.- The Activation of the Spermatozoon.- The Activation of the Egg.- Concluding Remarks.- References.- 3 Effects of Anti-Zona Pellucida Monoclonal Antibodies on Fertilization and Early Development.- Summary.- Monoclonal Antibodies.- Passive Immunization of Mice.- In Vitro Fertilization and Development.- Production of Monoclonal Antibodies.- Distribution of Zona Proteins.- Passive Immunization of Mice.- In Vitro Fertilization.- Reversibility of Contraception.- Effect on Development.- Discussion.- References.- 4 Nature of the Mouse Eggs Receptor for Sperm.- Summary.- Composition of the Mouse Zona Pellucida.- Identification of the Mouse Eggs Sperm Receptor: Experimental Approach.- Identification of the Mouse Eggs Sperm Receptor: Observations.- Identification of the Mouse Eggs Inducer of the Acrosome Reaction: Experimental Approach.- Identification of the Mouse Eggs Inducer of the Acrosome Reaction: Observations.- Structure of ZP3: The Mouse Eggs Receptor for Sperm.- Identification of the Molecular Nature of the Sperm Receptor Activity of ZP3.- Identification of the Molecular Nature of the Acrosome Reaction-Inducing Activity of ZP3.- Discussion.- References.- 5 The Receptor Function of Galactosyltransferase During Mammalian Fertilization.- Summary.- Cell Surface GalTase.- The T/t-Complex of the Mouse.- Surface GalTase on Mutant t-Sperm.- The Effects of In Vitro Capacitation on Surface GalTase Activity.- Perturbing Sperm GalTase Inhibits Sperm/Egg Binding.- Inhibition of Sperm/Zona Binding by Purified GalTase.- Inhibition of Sperm/Zona Binding by Monospecific Anti-GalTAse IgG.- Sperm GalTase Substrates in the Zona Pellucida.- Conclusions.- References.- 6 Immunological Identification of Sperm Antigens that Participate in Fertilization.- Summary.- Acrosomal Crescent Localization: MlS™