Molecular and Cellular Enzymology [Paperback]

Proteins constitute the working-class molecules of the cell. Hence, understanding the way they act is a prerequisite for understanding how a cell functions and how life evolves. Aspects such as the protein-ligand relationship, recognition, protein evolution by point mutation, enzyme-substrate interactions, behaviour of an enzyme in a living cell, control and dynamics of enzyme networks as well as the physico-chemical background of enzyme actions and multi-enzyme complexes are comprehensively treated in this volume.Proteins constitute the working-class molecules of the cell. Hence, understanding the way they act is a prerequisite for understanding how a cell functions and how life evolves. Aspects such as the protein-ligand relationship, recognition, protein evolution by point mutation, enzyme-substrate interactions, behaviour of an enzyme in a living cell, control and dynamics of enzyme networks as well as the physico-chemical background of enzyme actions and multi-enzyme complexes are comprehensively treated in this volume.Dynamics of Enzyme Reactions and Metabolic Networks in Living Cells. A Physico-Chemical Approach.- 1. Introduction.- 2. Flows and forces, diffusion, partition of mobile ions by charged matrices.- 3. Compartmentalization of enzyme reactions and the energy metabolism of the cell.- 4. Coupling between reactant diffusion and bound enzyme reaction rate.- 5. Electric partitioning of ions and reaction rate of bound enzyme systems.- 5.1. Electrostatic co-operativity of a bound enzyme system.- 5.2. Spatial organization of fixed charges and enzyme molecules as a source of co-operativity.- 6. An example of enzyme behaviour in organized biological systems: the dynamics of enzymes bound to plant cell walls.- 7. Control and dynamics of enzyme networks.- 7.1. Control of a linear metabolic network.- 7.2. Dynamic organization of a metabolic cycle in homogeneous phase.- 7.3. Dynamic organization of a metabolic cycle at the surface of a charged membrane.- 8. ControlÒ