The proteome consists of a complex mixture of proteins each of which need to be folded correctly in order to function for the health of the organism, and many of these proteins require molecular chaperones to reach the correct conformation and, in some cases, to remain in a folded form.? In Molecular Chaperones: Methods and Protocols, expert researchers address a wide variety of approaches to the study these mechanisms, featuring the workings of heat shock proteins and heat shock transcription factors, in vitro and in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters features introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Authoritative and cutting-edge,
Molecular Chaperones: Methods and Protocols serves as an ideal guide for all scientists who wish to pursue this vital biological action and its impact on human health and disease.
Written in the successful <i>Methods in Molecular Biology </i> series format, chapters include introductions to their respective topics, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
1. Targeted Deletion of Hsf1, 2, and 4 Genes in Mice
??????????? Xiongjie Jin, Binnur Eroglu, Demetrius Moskophidis, and Nahid F. Mivechi
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2. The Role Of Heat Shock Factors in Stress-Induced Transcription?????
??????????? Yue Zhang, Shiuh-Dih Chou, Ayesha Murshid, Thomas L. Prince, Sheila Schreiner, Mary Ann Stevenson, and Stuart K. Calderwood
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