Molecular Diagnostics For the Clinical Laboratorian [Hardcover]

Part I. IntroductionAn Historical Perspective on the Clinical Diagnostic LaboratoryRobert E. MoorePart II. Basic Molecular BiologyAn Overview of Nucleic Acid Chemistry, Structure, and Function: The Foundations of Molecular BiologyWilliam B. ColemanExtraction of Nucleic AcidsPaul N. Bogner and Anthony A. KilleenNucleic Acid Blotting Techniques: Theory and PracticeTerry Amiss and Sharon Collins PresnellThe Polymerase Chain ReactionWilliam B. Coleman and Gregory J. TsongalisBioinformatics: Computer-Based Approaches to Genetic AnalysisSharon L. RickettsPart III. Molecular Diagnostic TechnologiesPCR-Based Methods for Mutation DetectionIan M. Frayling, Emma Monk, and Rachel ButlerAlternative Methods for Amplified Nucleic Acid TestingDeborah A. Payne and Laurie E. SowerElectrophoretic Methods for Mutation Detection and DNA SequencingW. Edward Highsmith, Jr.Single-Nucleotide Polymorphisms: Testing DNA Variation for Disease AssociationUlrich Broeckel and Martin J. HessnerMicroarray Approaches to Gene Expression AnalysisDavid Neil Hayes and Matthew MeyersonMethods for Analysis of DNA MethylationAlexander DobrovicPart IV. Other Clinical Diagnostic TechnologiesFlow CytometryJoseph A. DiGiuseppeMedical CytogeneticsMartha B. KeagleFluorescence In Situ Hybridization: A Major Milestone in Luminous CytogeneticsSuneel D. Mundle and Robert J. KoskaImmunohistochemistryJohn Hunt, Larissa Davydova, Richard W. Cartun, and Maria BaiulescuLaser Capture MicrodissectionC. Robert Bagnell, Jr.Part V. Quality Assurance in the Molecular Diagnostics LaboratoryFramework for Quality Assurance in Molecular DiagnosticsMarlene Sabbath-Solitare, Selwyn J. Baptist, and Teresita Cuyegkeng RedondoVerification of Molecular AssaysBrent L. SeatonStandards and Standardization of Molecular DiagnosticsJohn P. Jakupciak and Catherine D. O'ConnellLaboratory-Developed Tests in Molecular DiagnosticsAndrea Ferreira-Gonzalez and Carleton T. GarrettPart VI. Applications of Molecular Diagnostics for Genetic DiseasesAn Olc,