Presenting topics from the basic application of molecular genetics to more complex gene expression analysis using different models of study, this detailed volume explores asthma through the lens of genetics, considered to play an essential role in the etiopathogenesis of the disease. Since asthma is a complex disease, this book is designed to provide a review of the most useful techniques with examples of their applications in specific laboratory protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Molecular Genetics of Asthma serves as an ideal guide to researchers investigating this vital field of study.
1. Applications of Molecular Genetics to the Study of Asthma
Catalina S. Sanz Lozano, Virginia Garc?a-Solaesa, Ignacio D?vila Gonz?lez, and Mar?a Isidoro-Garc?a
2. Assessment and Validation of New Genetic Variants: A systematic In Silico Approach Bel?n Garc?a Berrocal, Asunci?n Garc?a-S?nchez, and Mar?a Isidoro-Garc?a
3. Introduction to the Gene Expression Analysis Ignacio San Segundo-Val and Catalina S. Sanz Lozano
4. Real Time lcb