In recent years, molecular imaging techniques have grown to be invaluable tools for molecular biology research and, to a more modest extent, clinical medicine. In Molecular Imaging: Methods and Protocols, expert researchers explore the latest advances in the field, describing a rich variety of practical procedures and methods for diverging imaging technologies. Chapters are divided into sections that cover the imaging of basic molecular and biochemical events, imaging in pre-clinical settings, and imaging in clinical settings. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Comprehensive and ground-breaking, Molecular Imaging: Methods and Protocols is an essential handbook for students, established practitioners, and research fellows alike.
In recent years, molecular imaging techniques have grown to be invaluable tools. In this book, expert researchers explore the latest advances in the field, describing a rich variety of practical procedures and methods for diverging imaging technologies.
Molecular Imaging: Methods and ProtocolsEditor Khalid Shah, PhDTABLE OF CONTENTSTable of ContentsPrefaceContributorsImaging Biochemical Pathways and Molecular Events1.Bioluminescence resonance energy transfer (BRET) Imaging in Plant Seedlings and Mammalian CellsMohammed Soutto, Qiguang Xie, Xiaodong Xu, Yunfei Zhang, and Carl Hirschie Johnson2.Luciferase Protein Complementation Assays for Bioluminescence Imaging of Cells and MiceGary Luker and Kathryn Luker3.Hybrid Raman-Fluorescence Microscopy on Single Cells using Quantum DotsHenk-Jan van Manen and Cees Otto4.Labeling of Mesenchymal Stem Cells with Bioconjugated Quatum DotsBhranti S. Shah and Jeremy J. Mao5.Quantification of miRNA abundance in single cells using locked nucleic acid-FISH and enzyme lal³°