Molecular Typing of Blood Cell Antigens [Hardcover]

This volume aims to bring together a variety of protocols useful for DNA-based typing of blood cell antigens. Protocols range from simple approaches with low technical complexity to highly sophisticated modern developments. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Molecular Typing of Blood Cell Antigens summarizes contributions from leading scientist in the field DNA typing for blood cell antigens.

1. Validation Of Genotyping Protocols for Diagnostic Use

Paul Metcalfe

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2. High Resolution Melting Analysis Of Single Nucleotide Polymorphisms

Carol M. Bruzzone And Clifford J. Steer

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3. High-Speed Droplet-Allele-Specific Polymerase Chain Reaction for Genotyping Of Single Nucleotide Polymorphisms

Kazuyuki Matsuda And Takayuki Honda

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4. Blood Grouping Based On Pcr Methods and Agarose Gel Eletrophoresis

Ana Maria Sell And Jeane Eliete Laguila Visentainer

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5. Parallel Donor Genotyping For 46 Selected Blood Group and 4 Human Platelet Antigens Using High-Throughput Maldi-Tof Mass Spectrometry

Stefan Meyer, Nadine Trost, Beat M. Frey, And Christoph Gassner

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6. Pcr With Sequence-Specific Primers for Typing of Diallelic Blood Groups

Gabriele Rink, Erwin A. Scharberg, and Peter Bugert

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7. High-Resolution Melting Analysis for Genotyping Duffy Blood Group Antigens

Ewa Aukasik, Kazimiera Wa[niowska, Magdl³"