Morphogen Gradients Methods and Protocols [Hardcover]

This detailed book presents methods focusing on the visualization of morphogen gradients, the analysis of their biophysical and biological properties, and the theoretical aspects underlying their functions. The study of morphogen gradients combines biophysics, cell and developmental biology, and applied mathematics approaches to understand their formation, their biological functions, and the prediction of their behavior in space and time, and this volume serves as a practical, hands-on guide to the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Part I: Visualization of Morphogen Gradients

 

1. Analysis of Expression Gradients of Developmental Regulators in Arabidopsis thaliana Roots

            Mar?a Florencia Ercoli, Rodrigo Vena, Camila Goldy, Javier F. Palatnik, and Ramiro E. Rodriguez

 

2. Detection and Quantification of the Bicoid Concentration Gradient in Drosophila Embryos

            Feng He, Honggang Wu, David Cheung, and Jun Ma

 

3. Imaging Cytonemes in Drosophila Embryos

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