Mouse Embryogenesis Methods and Protocols [Hardcover]

This volume explores protocols for identifying mutant mice and characterizing parts of their anatomical, functional, cellular, and molecular phenotypes. The chapters in this book look at anatomical and functional phenotyping using quantitative imaging, isolation of specific embryonic cell types for cell culture, analysis of gene expression, and how to define chromatin structure. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Mouse Embryogenesis: Methods and Protocols is a valuable resource for experimentalists interested in discovering new aspects of embryogenesis control, organ function, and the origin of disease.

Preface&

Table of Contents&

Contributing Authors&

1. Mouse Genotyping

Sandra Vuong and Paul Delgado-Olguin

2. Visualizing the Vascular Network in the Mouse Embryo and Yolk Sac

Anna R. Roy and Paul Delgado-Olguin

3. In Vivo Evaluation of the Cardiovascular System of Mouse Embryo and Fetus Using High Frequency Ultrasound

Yu-Qing Zhou, Lindsay S. Cahill, and John G. Sled

4. Dynamic Imaging of Mouse Embryos and Cardiodynamics in Static Culture

Andrew L. Lopez, III and Irina V. Larina

5. In Vivo Imaging of the Mouse Reproductive Organs, Embryo Transfer, and Oviduct Cilia Dynamics Using OlS