Mouse Models for Drug Discovery Methods and Protocols [Paperback]

With genetic engineering, systems explored in this book now exist allowingfor the simple, efficient, and near universally precise genetic manipulationdirectly in any organism, including the mouse. Herein, these models are appliedto a wide field of disease areas, including diabetes, cardiovascular disease,skin disorders, cancer, neurodegenerative and neuromuscular diseases, retinaldisorders, as well as various behavioral models. Written for the highlysuccessful Methods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls. 

1. Genetically Defined Strains in DrugDevelopment and Toxicity Testing

            Michael F.W. Festing

 

2. Simple, Efficient CRISPR-Cas9Mediated Gene Editing in Mice: Strategies and Methods

            Benjamin E. Low, Peter M. Kutny, andMichael V. Wiles

 

3. Mouse Sperm Cryopreservation andRecovery of Genetically Modified Mice

            Benjamin E. Low, Rob A. Taft, andMichael V. Wiles

 

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