Mouse Molecular Embryology Methods and Protocols [Paperback]

In Mouse Molecular Embryology: Methods and Protocols, expert researchers in the field detail many of the protocols used to study? mouse embryology. ?These include protocols and techniques that? are close to the embryo : such as, manipulating embryonic gene expression, culturing explanted embryonic tissue and harvesting embryonic RNA.? With additional chapters?on fluorescence imaging, lineage tracing, and genetic ablation. Written in the highly successful Methods in Molecular Biology ?series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

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Authoritative and practical, Mouse Molecular Embryology: Methods and Protocols seeks to aid scientist in the further study of mouse embryo and its relation to other aspects of biological research.

This book details many of the protocols used to study?mouse embryology. It provides step-by-step detail essential for reproducible results and contains expert implementation advice.

1. In situ Hybridization Methods for Mouse Whole Mounts and Tissue Sections with and Without Additional ?-galactosidase Staining

Yoshihiro Komatsu, Satoshi Kishigami, and Yuji Mishina

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2. Two Color In Situ Hybridization of Whole-Mount Mouse Embryos

Kristin K. Biris and Terry P. Yamaguchi

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3. Detection and Monitoring of MicroRNA Expression in Developing Mouse Brain and Fixed Brain Cryosections

Davide De Pietri Tonelli, Yoanne M. Clovis and ?Wieland B. Huttner

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4. Laser Capture Microdissection of Embryonic Cells and PrelCÝ