Mouse Retinal Phenotyping Methods and Protocols [Hardcover]

The aim of this volume is to provide a wide range of techniques and aspects of mouse retinal phenotyping and characterization. Chapters guide readers through morphological and functional properties of the mouse retina and techniques for the analysis of outer to mid-retina and mid- to inner retina. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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Authoritative and cutting-edge, Mouse Retinal Phenotyping: Methods and Protocols aims to ensure successful results in the further study of this vital field.?

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Part I: Overview

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1??????????????? Morphological Survey from Neurons to Circuits of the Mouse Retina

Yoshihiko Tsukamoto

2??????????????? Measuring Retinal Function in the Mouse

Jan Kremers and Naoyuki Tanimoto

3??????????????? Modeling Retinal Diseases Using Genetic Approaches in Mice

Akiko Maeda and Tadao Maeda

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Part II: Outer to Mid-Retina

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4??????????????? Cell Culture Analysis of the Phagocytosis of Photoreceptor Outer Segments by Primary Mouse RPE Cells

Roni A. Hazim and David S. Williams

5??????????????? Two-photon Microscopy (TPM) and Fluorescence Lifetime Imaging Microscopy (FLIM) of Retinal Pigment Epithelium (RPE) of Mice In Vivo

Yoko Miura

6??????????????? RPE Visual Cycle and Biochemical Phenotypes of Mutant Mouse Models

Bhubanananda Sahu and Akiko Maeda