Mucins Methods and Protocols [Hardcover]

Epithelial mucins are large complex cell surface and secreted glycoproteins produced by mucosal epithelial cells.? In, Mucins: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study Mucins. These include methods and techniques? for the best approaches to analysing each specific area of mucin biochemistry, physiology and biophysics before providing individual detailed experimental protocols together with troubleshooting and interpretation tips. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

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Authoritative and practical, Mucins: Methods and Protocols is designed to be a useful resource for those entering the mucin field and to facilitate those already studying mucins to broaden their experimental approaches to understanding mucosal biology.

This volume presents an overview of the methods commonly used to study Mucins. These include the best approaches to analysing each specific area of mucin biochemistry, physiology and biophysics, in addition to detailed experimental protocols together with troubleshooting and interpretation tips.

1. Mucin Methods: Genes Encoding Mucins And Their Genetic Variation With A Focus On Gel Forming Mucins

?Karine Rousseau and Dallas M. Swallow

2. Gel-forming and Cell-associated Mucins  preparation for Structural and Functional Studies

?Julia R. Davies, Claes Wickstr?m and David J. Thornton

3. Detecting, Visualising and Quantifying Mucins

Ceri A. Harrop, David J. Thornton and Michael A. McGuckiló+