Our understanding of the molecular and cellular mechanisms that control skeletal muscle development, regeneration, and adaptive responses to activity has increased dramatically in recent years, fostered by innovative techniques and approaches that are either specifically designed or adapted for research in skeletal muscle biology.? Myogenesis: Methods and Protocols presents detailed, step-by-step methods in the study of the molecular and cellular biology of skeletal muscle cells.? Protocols from different model systems including mammalian, avian, zebrafish, and invertebrate skeletal muscle are included in this volume.? Highlighted topics cover a wide range of interests and expertise including myogenic and stem cell isolation, investigation of models of exercise and disuse, viral vector delivery systems, calcium imaging, cell profiling, as well as protein-DNA and protein-protein interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Comprehensive and authoritative,
Myogenesis: Methods and Protocols serves as an invaluable, state-of-the-art resource for experienced and emerging scientists in basic research as well as clinical and regenerative medicine.
This volume in the Methods in Molecular Biology series presents detailed, step-by-step methods for protocols used in the study of the molecular and cellular biology of skeletal muscle cells. Includes lists of materials and reagents, tips and pitfalls.
Part I: Isolation and Analysis of Skeletal Muscle Precursor Cells
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1. Isolation and Characterization of Human Fetal Myoblasts
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