Necrosis Methods and Protocols [Paperback]

Cell death is an essential process in development, and a major contributor to a wide range of human diseases. ?Three major classifications of cell death, apoptosis, autophagic cell death and necrosis, have been described for years, and the existence of many more forms of cell death is now accepted. In, Necrosis: Methods and Protocols experts in the field provide a wide range of methods and techniques for the study of necrosis in vitro and in vivo. These include methods and techniques for the analysis of necrosis in mammalian cells, characterization of alternative forms of cell death: entosis and pyroptosis, and analysis of cell death in non-mammalian model sytems and mammalian tissues, including chapters on skin, brain, and heart. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
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Authoritative and practical, Necrosis: Methods and Protocols describes techniques ?in an easy to follow manner, with details so that beginners can succeed with challenging techniques.This Methods in Molecular Biology book offers techniques for studying necrosis in vitro and in vivo, including methods for analyzing necrosis in mammalian cells, alternative forms of cell death: entosis and pyroptosis, and chapters on skin, brain and heart.

Part I: Analysis of Necrosis In Vitro

1. Navigation to the Graveyard- Induction of Various Pathways of Necrosis and their Classification by Flow Cytometry

Christina Janko, Luis Munoz, Ricardo Chaurio, Christian Mauer?der, Christian Berens, Kirsten Lauber, Martin Herrmann

?2. Time-llÓï