The discovery of stem and progenitor cells in the adult mammalian CNS challenged the long standing no new neuron doctrine and opened the door to the potential for cell replacement therapy. The process from discovery to clinical applications can be long and tortuous, requiring rigorous steps involving standardized and precise protocols. Neural Progenitor Cells: Methods and Protocols is a collection of practical articles describing techniques used to study neural stem and progenitor cells. The volume also highlights the promise of stem cell-based therapeutic applications for CNS disorders. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
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Authoritative and easily accessible, Neural Progenitor Cells: Methods and Protocols serves both professionals and novices with its well-honed methodologies.
This volume details techniques used to study neural stem and progenitor cells as well as highlights the promise of stem cell-based therapeutic applications for CNS disorders. It features step-by-step, readily reproducible protocols and implementation advice.
Part I- Culturing Neural Precursor Cells from Primary Tissue
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1. Culturing Fetal Precursors Cells Using Free-Floating Serum-Free Conditions
??????????? Andrew Chojnacki and Samuel Weiss
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2. In vitro Expansion of Foetal Neural Progenitors as Adherent Cell Lines
??????????? Steven M. Pollard
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3. Isolate and Culture Precursor Cells from the Adult Periventricular Area
??????????? Chiara Cavazzin, Margherita Neri, and Anl"
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