Neuroanatomical Tract-Tracing Methods [Paperback]

1 Experimental Neuroanatomy: General Approaches and Laboratory Procedures.- I. Introduction.- II. Tract-Tracing Methods.- A. Types of Methods.- B. Choice of Method.- III. Practical Problems.- A. The Experimental Animal.- B. The Tissue.- IV. Analysis.- A. Amount of Material.- B. Analytical Approach.- C. Normal Material.- D. Mapping and Reconstruction.- V. The Neuroanatomical Laboratory.- A. Animal Quarters and Surgery.- B. Optical Equipment.- C. Laboratory Precautions.- VI. Appendix.- A. Encasing of Tissue for Preparation of Frozen Sections.- B. Preparation of Frozen Sections on Sliding Microtome.- C. Special Staining Procedures.- D. Graphic Reconstructions.- References.- 2 Methods for Selective, Restricted Lesion Placement in the Central Nervous System.- I. Introduction.- II. Stereotaxic Technique.- A. Theoretical Background.- B. The Stereotaxic Atlas.- C. Verification of the Coordinates.- III. Nonselective Lesion Techniques.- A. Mechanical Lesions.- B. Injected, Nonselective Toxins.- C. Alterations of Cerebral Vasculature.- D. Radioisotopic and Related Methods.- E. Ultrasound Lesions.- F. Thermal Lesions.- G. Electrolytic Lesions.- IV. Evaluation of the Electrolytic Lesion.- V. Selective Lesion Techniques.- A. Kainic Acid and Glutamate Derivatives.- B. Neurotoxic Catecholamine and Indolamine Derivatives.- VI. The Interpretation of Lesion Effects.- VII. Appendix: Stereotaxic Atlases.- A. Rat Brain Atlases.- B. Cat Brain Atlases.- C. Primate Brain Atlases.- D. Dog Brain Atlases.- References.- 3 Methods for Delivering Tracers.- I. Introduction.- II. Pressure Injection.- A. Microsyringe Injection.- B. Micropipette Injection.- III. Iontophoretic Injection.- A. General Considerations.- B. Extracellular Injection.- C. Intracellular Injection.- IV. Appendix.- A. Microelectrode Preparation.- B. Iontophoresis Assays.- References.- 4 Silver Methods for the Impregnation of Degenerating Axoplasm.- I. Introduction.- II. Theoretical Considerations.- A. When to Use the Silver MethlÃ6