New Insights into Cell and Membrane Transport Processes [Paperback]

I. Na+, K+, and H+ Transport.- 1 Molecular Properties of Voltage-Sensitive Sodium Channels.- 1. Introduction.- 2. Physiological Properties of Sodium Channels.- 3. Neurotoxins as Molecular Probes of Sodium Channels.- 4. Identification of Protein Components of Sodium Channels in Neurons.- 5. Molecular Size of the Sodium Channel.- 6. Protein Subunits of the Purified Sodium Channel.- 7. Reconstitution of Sodium Channel Function from Purified Components.- 8. The Amino Acid Sequence of the Sodium Channel as Inferred from cDNA Clones.- References.- II. Proton Pumps and Electrochemical Gradients.- 2 Studies of a Biological Energy Transducer: The lac Permease of Escherichia coli.- 1. Introduction.- 2. Morphology and Permeability of Proteoliposomes Reconstituted with lac Permease.- 3. A Single Polypeptide is Required for Lactose Transport.- 4. Reconstitution of Active Transport in Proteoliposomes Containing lac Permease and Cytochrome o Oxidase.- 5. Secondary Structure of lac Permease.- 6. Immunologic Reagents as Structure/Function Probes.- 7. Subunit Structure.- 8. Oligonucleotide-Directed Site-Specific Mutagenesis.- References.- 3 The Erythrocyre Anion-Exchange Protein: Primary Structure Deduced from the cDNA Sequence and a Model for Its Arrangement within the Plasma Membrane.- 1. Overview.- 2. Introduction.- 3. Materials and Methods.- 3.1. Isolation of cDNA Clones and DNA Sequencing.- 3.2. Computer-Assisted DNA Sequence Analysis.- 3.3. Cell Culture.- 3.4. RNA Blot Hybridization.- 4. Results.- 4.1. Mouse and Human Band 3 Share Sequence Homology.- 4.2. Band 3 Has Three Structural Domains.- 4.3. Band 3 Expression Is Regulated during Differentiation of MEL Cells.- 5. Discussion.- 5.1. Transmembrane Orientation of the Band 3 Polypeptide.- 5.2. Location of the H2DIDS-Binding Lysines in Band 3.- 5.3. The Structure and Mechanism of the Anion-Exchange Site.- References.- 4 The Lysosomal Proton Pump.- 1. Introduction.- 2. ATP-Dependent Acidification of Lysosomes.- 3. Characteristicsl³ã