NF-kappa B Methods and Protocols [Hardcover]

Focusing on essential insight into the methods and techniques required to dissect the complex mechanisms of NF-?B activation, regulation, and function.?NF-kappa?B: Methods and Protocols?guides readers through standard approaches to detect NF-?B pathway activation, detection and analysis of?NF-?B signaling, and methods to study the control of?NF-?B?signaling. As a volume in the highly successful Methods in Molecular Biology?series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, NF-Kappa?B: Methods and Protocols?provides a timely and invaluable resource for researchers seeking to perform experiments aimed at understanding the role of NF-?B signaling in health and disease.

Part I: Standard Approaches to Detect NF-?B Pathway

1. Electrophoretic Mobility Shift Assay Analysis of NF-kkB DNA Binding

Sitharam Ramaswami and Matthew S. Hayden M.D

?2. Detection of IkB Degradation Dynamics and IkB-a Ubiquitination

Petro Starokadomskyy and Ezra Burstein

?3. Measurement of NF-?B Transcriptional Activity and Identification of NF-?B cis-regulatory Elements using Luciferase Assays

Patricia E. Collins, Christine OCarroll, and Ruaidhr? J. Carmody

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?Part II: Detection and Analysis of NF-?B Signaling

4. Assessing Sites of NF-?B DNA Binding using Chromatin Immunoprecipitation

Amy Colleran, Patricia E. Collins, and Ruaidhr? J. Carmody

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5. IKK Kinase Assay for Assessment of Canonical NF-kB Activation in Neurons