Nonconventional Yeasts in Biotechnology A Handbook [Paperback]

This is the first book to extensively and exclusively cover nonconventional yeasts - all yeasts other than S. cerevisiae and S. pombe. In addition to useful background information, the author includes detailed protocols allowing the investigation of basic and applied aspects for a wide range of these organisms.
Due to the increasing importance of nonconventional yeasts in biotechnological applications, this book should become the standard reference for both pure and applied scientists working in the fields of microbiology and biochemistry.Nonconventional yeasts - all yeasts other than S. cerevisiae and S. pombe - are attracting increasing attention in basic research and biotechnological applications. Due to their exceptional metabolic pathways, they have been used in various biotechnological processes for producing foods or food additives, drugs or a variety of biochemicals.
This book is the first to extensively cover nonconventional yeasts. In addition to useful background information detailed protocols are included, allowing investigation of basic and applied aspects of a wide range of nonconventional yeast species.1 Principles and Methods Used in Yeast Classification, and an Overview of Currently Accepted Yeast Genera.- 1 Introduction.- 2 Some Principles of Yeast Taxonomy.- 3 Trends in the Systematics of Yeasts.- 4 Phylogeny.- 5 Methods.- 5.1 Morphology.- 5.1.1 Vegetative Morphology.- 5.1.2 Generative Morphology.- 5.2 Physiological Characterization of Yeasts.- 5.2.1 Fermentation Tests.- 5.2.2 Assimilation Tests.- 5.2.3 Vitamin Requirements.- 5.2.4 Other Tests.- 5.3 Mating.- 5.3.1 Ascomycetes.- 5.3.2 Basidiomycetes.- 5.4 Nuclear Staining.- 5.4.1 Staining Nuclei Using DAPI.- 5.4.2 Staining Nuclei with Propidium Iodide.- 5.4.3 Staining Nuclei with Mithramycin and Ethidium Bromide.- 5.4.4 Staining Nuclei with Giemsa.- 5.5 DNA.- 5.5.1 Isolation.- 5.5.2 Analysis of Base Composition.- 5.5.3 Hybridization of Nuclear DNA.- 5.5.4 Amplification of Yeast DNló.