Notch Signaling Methods and Protocols [Hardcover]

Notch Signaling: Methods and Protocols focuses on molecular, biochemical and bioinformatics aspects of Notch signaling. Chapters analyze the Notch interactome, post-translational modifications of Notch, ligand binding assays, methods to assess proteolytic cleavage and transcriptional targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Notch Signaling: Methods and Protocols is a valuable contribution to hundreds of labs and thousands of scientists who pursue this research area with vigor.

1. Introduction to Notch Signaling

Shinya Yamamoto, Karen L. Schulze, and Hugo J. Bellen

2. Genetic Screens to Identify New Notch Pathway Mutants in Drosophila

Nikolaos Giagtzoglou

3. Structure-function Analysis of Drosophila Notch using Genomic Rescue Transgenes

Jessica Leonardi and Hamed Jafar-Nejad

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4. Overview of Genetic Tools and Techniques to Study Notch Signaling in Mice

Thomas Gridley and Andrew K. Groves

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5. Immunohistochemical Tools and Techniques to Visualize Notch in Drosophila melanogaster

Emiliana Tognon and Thomas Vaccari

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6. Antibody Uptake Assay and in vivo Imaging to Study Intracellular Trafficking of Notch and Delta in Drosophila

Lydie Couturier and Fran?ois Schweisguth

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7. Tracl3,