Nuclear Reprogramming: Methods and Protocols, Second Edition includes not only classic methods to perform nuclear transfer in different species but also several techniques to assess the early and late development of the reconstructed embryos, at the cellular, molecular, and epigenetic level. Over the past several years, many technical improvements have been made to improve somatic cell nuclear transfer (SCNT) efficiency, all of which are reflected in the detailed chapters of this fully revised collection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date,
Nuclear Reprogramming: Methods and Protocols, Second Edition will be of interest not only to cloners but also to researchers concerned with studying the development of embryos.
1. Nuclear Transfer in the Mouse
??????????? Vincent Brochard and Zichuan Liu
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2. Nuclear Transfer in the Rabbit
??????????? Nathalie Daniel and Patrick Chesn?
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3. Nuclear Transfer in Ruminants
??????????? Joon-Hee Lee and Walid E. Maalouf
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4. Nuclear Transfer and Transgenesis in the Pig
??????????? Mayuko Kurome, Barbara Kessler, Annegret Wuensch, Hiroshi Nagashima, and Eckhard Wolf
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5. Embryonic Stem Cell-Somatic Cell Fusion and Post-Fusion Enucleation
??????????? Huseyin Sumer and Paul J. Verma
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6. Analysis of Nuclear Reprogramming Following Nuclear Transfer to Xenopus Oocyte
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