Nucleic Acid Aptamers Selection, Characterization, and Application [Paperback]

This volume provides protocol references covering recent developments in the aptamer field. Within the last decade, aptamers have become more and more popular, and their sophisticated biophysical properties together with their ability to be easily modified and, thus, adapted to various regimens makes them a very promising class of compounds. Divided into three sections, the book covers selection, a series of analytical methods to assess biophysical properties of aptamer-target interactions, as well as various applications of aptamers. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and easy to follow, Nucleic Acid Aptamers: Selection, Characterization, and Application provides a state-of-the-art summary of recent developments in the aptamer field and will be a helpful resource for scientists in the life sciences working with aptamers as tools to elucidate biological systems.

Part I: Selection

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1. Selection of Aptamers for Metabolite Sensing and Construction of Optical Nanosensors

??????????? Yi Long, Franziska Pfeiffer, G?nter Mayer, Tine Daa Schr?der, Veli Cengiz ?zalp, and Lars Folke Olsen

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2. SELEX of Cell-Specific RNA Aptamers

??????????? Katharina Berg, Eileen Magbanua, and Ulrich Hahn

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3. Developing Aptamers by Cell-Based SELEX

??????????? Silvia Catuogno, Carla Lucia Esposito, and Vittorio de Franciscis

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4. DNA Aptamer Generation by Genetic Alphabet Expansion SELEX (ExSELEX) Using an Unnatural Base Pair System

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