Nucleic Acid Detection Methods and Protocols [Paperback]

In Nucleic Acid Chemistry: Methods and Protocols, expert researches in the field detail techniques and approaches for the detection of DNA and RNA. These techniques include the recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, new application of isothermal amplification techniques, assays with visual or electric signals for point-of-care diagnostics, improvement of fluorescent in situ hybridization, and new signal amplification techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

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Authoritative and practical, Nucleic Acid Chemistry: Methods and Protocols seeks to aid scientists in the further study of detection for DNA and RNA.

Part I: Advances in Sample Preparation

1. Getting Blood from a Stone: Ultra-Sensitive Forensic DNA Profiling of Microscopic Bio-particles Recovered from Touch DNA Evidence

Erin K. Hanson and Jack Ballantyne

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2. Detecting MicroRNA in Human Cancer Tissues with Fluorescence in situ Hybridization

Zonggao Shi, Jeff J. Johnson, and M. Sharon Stack

?Part II: New Real-Time and Instantaneous Assays

3. MN Azyme qPCR: A Superior Tool for Multiplex qPCR

Elisa Mokay and Alison V. Todd

4. A New, Multiplex, Quantitative Real-time l³«