The Nucleic Acid Protocols Handbook [Hardcover]

Part I. Nucleic Acid Extraction. Isolation of High-Molecular-Weight DNA from Animal Cells, Ian Garner. Isolation of mRNA by Affinity Chromatography, Sian Bryant and David L. Manning. Isolation and Purification of DNA from Plants, Justin Stacey and Peter G. Isaac. Purification of Uncontaminated, Intact Plant RNA, Shu-Hua Cheng, Brandon D. Moore, and Jeffrey R. Seemann. An Improved Method to Isolate Mitochondrial RNA from Green Plant Tissue, Fei Ye and Ralf Reski. Isolating Chromosomal DNA from Bacteria, Elisabeth Chachaty and Patrick Saulnier. Bacterial DNA Extraction for Polymerase Chain Reaction and Pulsed-Field Gel Electrophoresis, Elisabeth Chachaty and Patrick Saulnier. Isolation of Fungal Nucleic Acids, Surapareddy Sreenivasaprasad. Total RNA Isolation from Bacteria, John Heptinstall. Simultaneous RNA and DNA Extraction from Biopsy Material, Culture Cells, Plants, and Bacteria, Udo D?bbeling. Spectrophotometric Analysis of Nucleic Acids, John Heptinstall and Ralph Rapley. Part II. Basic Separation and Analysis of DNA. Restriction Endonuclease Digestion of DNA, Duncan R. Smith. Agarose Gel Electrophoresis of Nucleic Acids, D. Ross Williams and Ralph Rapley. Preparation of RNA Dot Blots, Rachel Hodge. Native Polyacrylamide Gel Electrophoresis, Adrian J. Harwood. Southern Blotting of Agarose Gels by Capillary Transfer, Ralph Rapley and Jane Davenport -Jones. Pulsed-Field Gel Electrophoresis, John Maule. HPLC of DNA and PCR Products, Elena D. Katz. Part III. Probe Design, Synthesis, and Labeling. End-Labeling of DNA Fragments, Adrian J. Harwood. Nick Translation and Random Hexamer Labeling of DNA, Jane Davenport-Jones. Generation of Labeled Probes by Polymerase Chain Reaction, Y. M. Dennis Lo and Shu F. An. Nonradioactive Oligonucleotide Probe Labeling, Sue Fowler and Ian Durrant. Preparation of Direct, Enzyme-Labeled DNA Probes, Ian Durrant and Timothy Stone. Random Prime Labeling of DNA Probes with Fluorescein-Tagged Nucleotides, Bronwen M. Harvey, Claire B.Wheellƒ