Nucleic Acid Therapeutics in Cancer [Paperback]

A cutting-edge review of the important issues underlying the therapeutic use of nucleic acid-mediated gene silencing. Topics range from basic methodology and delivery to targeting and clinical targets. The authors thoroughly explain the latest developments in RNA biology, as well as the underpinnings of RNA interference, oligodeoxynucleotide delivery into cells, and strategies for targeting these molecules to accessible regions within the mRNA. They also provide some examples of how these new therapeutic compounds are being used clinically.A cutting-edge review of the important issues underlying the therapeutic use of nucleic acid-mediated gene silencing. Topics range from basic methodology and delivery to targeting and clinical targets. The authors thoroughly explain the latest developments in RNA biology, as well as the underpinnings of RNA interference, oligodeoxynucleotide delivery into cells, and strategies for targeting these molecules to accessible regions within the mRNA. They also provide some examples of how these new therapeutic compounds are being used clinically.Part I. Introduction and PerspectiveAntisense Methodology: An Assessment After 25 YearsFritz EcksteinNucleic Acid Therapeutics: An IntroductionAlan M. GewirtzPart II. Basic MethodologyTargeted Genome Modification Via Triple Helix FormationFaye A. Rogers and Peter M. GlazerTherapeutic Applications of RibozymesJohn J. RossiUse of Catalytic DNA in Target Validation and TherapeuticsLun-Quan SunTargeted Destruction of Small, Stable RNAs: Principles Applicable to Antisense TherapiesDavid A. Dunbar and Susan J. BasergaMechanism of Action of Antisense RNA in Eukaryotic CellsZuo Zhang and Gordon G. CarmichaelPart III. DeliveryThe Transport of Oligonucleotides Into CellsR. L. JulianoPeptide-Mediated Delivery of Antisense Oligonucleotides and Related MaterialEric Viv?s, Jean Philippe Richard, and Bernard LebleuMolecular Vectors for Gene Delivery to Cancer CellsGuy Zuber, Jean-Serge Remy, Patrick Erbacher, lSe