Nucleic Acids in the Environment [Paperback]

Molecular biological techniques such as DNA/RNA extraction and purification, and especially the polymerase chain reaction, PCR, are rapidly gaining interest also in related fields, such as microbiology or environmental sciences. They offer new approaches and opportunities for the determination of microbial cells, DNA and RNA from soils, roots, rhizospheres, sediments and aquatic environments.
Detailed protocols for these applications are described in this manual.Molecular biological techniques such as DNA/RNA extraction and purification, and especially the polymerase chain reaction, PCR, are rapidly gaining interest also in related fields, such as microbiology or environmental sciences. They offer new approaches and opportunities for the determination of microbial cells, DNA and RNA from soils, roots, rhizospheres, sediments and aquatic environments.
Detailed protocols for these applications are described in this manual.1 Introduction to Nucleic Acids in the Environment: Methods and Applications.- 1.1 Introduction.- 1.2 Cell Lysis, Nucleic Acid Extraction and Purification Protocols.- 1.3 Analysis of Environmental Nucleic Acids.- 1.4 Concluding Remarks.- References.- 2 Recovery of Bacterial Cells from Soil.- 2.1 Introduction.- 2.2 Strategies for Cell Extraction.- 2.3 Recommended Methods.- 2.4 Practical Experiences and Recommendations.- References.- 3 Extraction, Purification, and Analysis of DNA from Soil Bacteria.- 3.1 Introduction.- 3.2 Bacterial DNA.- 3.3 Direct Extraction of DNA from Soil.- 3.4 Purity Control and Characterization of Soil Bacterial DNA.- References.- 4 Extraction and Analysis of Microbial DNA from Soil.- 4.1 Introduction.- 4.2 DNA Extraction Methods.- 4.3 Evaluation of DNA Extraction Methods.- 4.4 Methods for Analysis of DNA from Soil.- 4.5 Concluding Remarks.- References.- 5 Extraction and Amplification of DNA from the Rhizosphere and Rhizoplane of Plants.- 5.1 Introduction.- 5.2 Sample Preparation.- 5.3 Extraction, PurificatilS'